20×DAB显色液 A液和B液各3ml

DAB显色试剂盒(20X)


DAB显色试剂盒（20×）说明书
货号：DA1010




规格： DA1010-3 DA1010-10 溶液 A: 3ml 10ml 溶液 B： 3ml 10ml 保存： -20℃避光密闭保存，一年有效。避免反复冻融。短期可2-8℃保存。产品简介： 二氨基联ben胺（Diaminobenzidine,DAB）是过氧化物酶（POD）的底物，DAB显色液主要用于免疫过氧化物酶法，适用于辣根过氧化物酶HRP标记的免疫印记或免疫组化反应。过氧化物酶使底物发生反应，于反应部位产生棕色沉淀物。免疫印记可直接在膜上显示条带。免疫组化标本显色时间一般为室温5-20分钟，随后在显微镜下观察显色情况，显色充分后应将标本及时脱水封固。其终产物可直接在光镜下观察，也可经OsO4处理后，增加反应产物的电子密度，用于电镜观察。操作说明：
1. 对于组织切片或蛋白质印记膜，在与辣根过氧化物酶（HRP）标记的抗体或其它形式的探针孵育后，用适当洗涤液洗涤3-5次，每次3-5分钟。
2. 取900ul PBS（或双蒸水），加入50ul溶液A，50ul溶液B混合均匀，即配成DAB工作液。 如需要更大体积工作液，可按比例放大。此溶液必须现用现配，配好后避光保存，1小时内使用，过期后请将剩余的液体废弃。3. 向组织切片或膜上加入适量DAB工作液，确保能充分覆盖样品。
4. 室温避光孵育3-10 分钟或更长时间，避免光照，直至显色至预期深浅。
5. 去除DAB染色工作液，用蒸馏水洗涤2-3次即可终止显色反应。
6. 对于组织切片或细胞样品，显色反应终止后，可对其进行其他染料复染。对于膜，显色反应终止后，可以室温晾干避光保存。注意事项：
1．DAB溶液应低温密封保存。如有结晶析出，应确保结晶完全溶解再行使用；
2．显色工作液应现用现配，新鲜配制的工作液应为无色或浅棕色，如颜色过深，请勿使用；
3．显色时间严格控制，根据情况调整，以免显色过度。固相膜显色数小时后即会褪色，不能永久存在；
4．DAB为可疑致癌物，请采取必要的防范措施。操作时应戴手套，尽量避免与皮肤接触，用后及时彻底冲洗，接触DAB的实验用品最好经洗液浸泡24h后使用。
相关产品：
P1032 20×PBS， PH7.2-7.4
G1140 Mayer'苏木素染液(免疫组化)
A2010 AEC底物显色试剂盒

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