AOAC Method 996.11 AACC Method 76-13.01 RACI Standard Method for the measurement of total starch in cereal flours and food products. AACC and AOAC approved method. This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0).
INTRODUCTION:
Sulphur dioxide (SO2) is used widely as an additive in various forms,
most commonly as sulphites (or sulfites), in the wine, beverage
and food industries where it acts primarily as an antimicrobial and
antioxidant preservative.
During wine making, sulphites are used as an essential additive, usually
at post-malolactic fermentation, in the control of contamination
by Brettanomyces during aging and to also protect the wine against
detrimental “oxidative and enzymatic browning”. SO2 is only active
as an antimicrobial and antioxidant preservative in the unbound
“free” form. Given that SO2 becomes “inactive” when it binds the
colour pigments of wine, and with legal restrictions on SO2 levels in
wine, it has become valuable to wine producers to measure both the
Free SO2 (FSO2) and Total SO2 (TSO2). In addition to this, due to
the increased awareness of the adverse effects of sulphites and the
prevalence of sulphite intolerance in some individuals, sulphite levels
in foods and drinks are strictly regulated by various governing bodies
and therefore there is a requirement for accurate determination of
the level of sulphites in foods, beverages and wines.
This kit (K-TSULPH) is suitable for the specific measurement of
“Total Sulphite” in wines, beverages, foodstuffs and other materials.
KITS:
Kits suitable for performing 80 assays in manual format (or 800 assays
in auto-analyser format) are available from Megazyme. The kits
contain the full assay method plus:
Bottle 1: (2x) Total Sulphite Reagent 1 (40 mL)
Contains sodium azide (0.05 % w/v) as a preservative.
Stable for > 18 months at room temperature.
Bottle 2: (2x) Total Sulphite Reagent 2 (20 mL)
Contains sodium azide (0.05 % w/v) as a preservative.
Stable for > 18 months at room temperature.
Bottle 3: Sulphite Standard
Sodium Sulphite (5 g).
Stable for > 5 years at room temperature.