For the specific assay of succinic acid in wine, cheese, eggs, sauce and other food products.
INTRODUCTION:
Succinic acid is found in all plant and animal materials as a result of
the central metabolic role played by this dicarboxylic acid in the
Citric Acid Cycle. Succinic acid concentrations are monitored in the
manufacture of numerous foodstuffs and beverages, including wine,
soy sauce, soy bean flour, fruit juice and dairy products (e.g. cheese).
The ripening process of apples can be followed by monitoring the
falling levels of succinic acid. The occurrence of > 5 mg/kg of this
acid in egg and egg products is indicative of microbial contamination.
Apart from use as a flavouring agent in the food and beverage
industries, succinic acid finds many other non-food applications, such
as in the production of dyes, drugs, perfumes, lacquers, photographic
chemicals and coolants.
KITS:
Kits suitable for performing 20 assays in manual format (or 270 assays
in auto-analyser format or 200 assays in microplate format) are available
from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (8 mL, pH 8.4) plus sodium azide (0.02% w/v) as
a preservative.
Stable for > 2 years at 4°C.
Bottle 2: (x2) NADH plus stabiliser.
Stable for > 2 years at -20°C.
Bottle 3: (x2) ATP plus PEP and CoA.
Stable for > 2 years at -20°C.
Bottle 4: Pyruvate kinase plus L-lactate dehydrogenase suspension,
0.55 mL.
Stable for > 2 years at 4°C.
Bottle 5: Succinyl-CoA synthetase suspension (0.55 mL).
Stable for > 2 years at 4°C.
Bottle 6: Succinic acid (~ 2 g).
Stable for > 2 years at 4°C.