For the measurement of maltose, sucrose and D-glucose in plant and food products.
INTRODUCTION:
Maltose, sucrose and D-glucose are found in most plant and food
products. In plant materials D-glucose occurs as a free sugar in
sucrose, and in a range of oligosaccharides (galactosyl-sucrose
oligosaccharides and fructo-oligosaccharides) and polysaccharides, such
as starch and 1,3-1,4-β-D-glucans. Maltose (1,4-α-D-glucobiose) is
produced on hydrolysis of starch by α-amylase and β-amylase. In some
food products, these three sugars occur in admixture.
KITS:
Kits suitable for performing 30 determinations of maltose, sucrose and
D-glucose are available from Megazyme. The kits contain the full assay
method plus:
Bottle 1: Buffer (25 mL, pH 7.6) plus sodium azide (0.02 % w/v)
as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: NADP+ plus ATP.
Stable for > 5 years at -20°C.
Bottle 3: Hexokinase plus glucose-6-phosphate dehydrogenase
suspension, 2.25 mL.
Stable for > 2 years at 4°C.
Bottle 4: β-Fructosidase in sodium citrate buffer (pH 4.6),
lyophilised powder.
Stable for > 2 years at -20°C.
Bottle 5: α-Glucosidase in sodium citrate buffer (pH 6.6),
lyophilised powder.
Stable for > 2 years at -20°C.
Bottle 6: D-Glucose standard solution (5 mL, 0.4 mg/mL).
Stable for > 2 years at room temperature.