L-苹果酸[AF法]检测试剂盒 L-Malic Acid (

产品货号：K-LMALAF

品牌：Megazyme

产品品名：L-苹果酸[AF法]检测试剂盒

英文品名：L-Malic Acid (Analyser Format) Assay Kit

规格型号：50 assays (manual) / 500 assays (microplate) / 450 assays (auto-analyser)

245.5 mL of prepared reagent (e.g. 1 assays of 0.22 mL)1116 Assays

INTRODUCTION:

Some kits sold for manual enzymatic analysis of L-malic acid can’t be used effectively in an auto-analyser format, as they either lack linearity at higher substrate concentrations, on-machine stability, or the reagents are not present in optimal ratios, leading to significant wastage and thus increased cost per analysis. This kit has, however, been specifically developed for auto-analyser applications, and offers linearity up to 80 μg/mL of L-malic acid (in the final reaction solution), excellent on-machine stability and contains optimal amounts of each reagent. Polyvinylpyrollidone (PVP) has also been incorporated into the assay format to prevent performance inhibition by polyphenolic compounds (tannins). At the same time, the biochemical principle behind this assay format remains the same as all other L-malic acid kits (see equations 1 and 2 below).

KITS:

Kits suitable for the preparation of 245.5 mL of reagent (equivalent to 1116 reactions of 0.22 mL) are available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (48 mL, pH 10.0) plus L-glutamate and sodium azide (0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: (x2) NAD+ plus PVP.
Stable for
> 5 years at -20°C (freeze dried powder).
Dissolve the contents of bottle 2 in 12 mL of distilled water: Stable for > 1 year at 4°C or stable for > 2 years at -20°C (to avoid repetitive freeze / thaw cycles, divide into appropriately sized aliquots and store in polypropylene tubes).
Do not dissolve the contents of the second bottle until required.
Bottle 3: Glutamate-oxaloacetate transaminase suspension (2.5 mL).
Stable for
> 2 years at 4°C.
Bottle 4: L-Malate dehydrogenase suspension (2.5 mL).
Stable for
> 2 years at 4°C.

温馨提示：需要详细的产品使用说明书，请联系甄准生物客服人员，我们将竭诚为您服务。

甄准，甄心倾听您每一个标准！