葡甘露聚糖检测试剂盒 DGlucomannan Assay

产品货号：K-GLUM

品牌：Megazyme

产品品名：葡甘露聚糖检测试剂盒

英文品名：DGlucomannan Assay Kit

规格型号：50 Assays per kit

For the measurement of Glucomannan in plant products and food.

INTRODUCTION:

Glucomannan is a linear polysaccharide comprising 1,4-β-linked
D-glucosyl and D-mannosyl residues, where the glucose : mannose (Glc:Man) ratio varies considerably. Konjac (Amorphophallus konjac) glucomannan has a Glc:Man ratio of approx. 2:3, Aloe vera glucomannan a ratio of 1:2, and salep (Orchis mureo) glucomannan has a ratio of approximately 1:4. All of the polysaccharides are acetylated, which imparts water solubility. Glucomannans are major cell-wall reserve carbohydrates in tubers of several plant genera. At certain stages in the development of the plants, the glucomannan is degraded and serves as an energy source. The only industrially exploited glucomannan is konjac. Like carob galactomannan, this polysaccharide interacts with agar, carrageenan and xanthan to form solutions of increased viscosity or gels. This interaction is exploited in the pet-food industry in canned dog food. Recently, glucomannan has also been used in jelly sweets. In this application, the glucomannan has been found to be dangerous, leading to choking deaths of some consumers. This has led to a ban of these types of sweets in Europe and America, and a need for an accurate analytical procedure for glucomannan analysis.

KITS:

Kits suitable for performing 50 determinations of glucomannan are available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (25 mL, pH 4.5) plus sodium azide
(0.02% w/v) as
a preservative.
Stable for > 2 years at 4°C.
Bottle 2: Buffer (25 mL, pH 7.6) plus magnesium chloride and sodium azide (0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 3: NADP+ plus ATP.
Stable for
> 5 years at -20°C.
Bottle 4: β-Mannanase suspension (1.2 mL).
Stable for
> 2 years at 4°C.
Bottle 5: β-Glucosidase plus β-mannosidase suspension, 1.1 mL.
Stable for
> 2 years at 4°C.
Bottle 6: Hexokinase plus glucose-6-phosphate dehydrogenase suspension, 1.1 mL.
Stable for
> 2 years at 4°C.
Bottle 7: Phosphoglucose isomerase plus phosphomannose isomerase suspension, 1.1 mL.
Stable for
> 2 years at 4°C.
Bottle 8: D-Glucose plus D-mannose standard solution
(0.2 mg/mL
of each).
Stable for > 2 years at 4°C.

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