A simple procedure for the rapid and reliable measurement of glucose oxidase activity in industrial enzyme preparations and bread improver mixtures.
INTRODUCTION:
Glucose oxidase (GOX) catalyses the oxidation of β-D-glucose to
D-glucono-δ-lactone and hydrogen peroxide. It is highly specific for
β-D-glucose and does not act on α-D-glucose.
A major use of glucose oxidase has been in the determination of free glucose in bodyfluids, food and agricultural products. However, it has been gaining increasing attention in the baking industry; its oxidising effects result in a stronger dough. In some applications it can be used to replace oxidants, such as bromate and L-ascorbic acid. Other uses of glucose oxidase include the removal of oxygen from food packaging and removal of D-glucose from egg white to prevent browning.
KITS:
Kits suitable for performing 200 assays in manual format (or 1960
assays in auto-analyser format or 2000 assays in microplate format)
are available from Megazyme. The kits contain the full assay method
plus:
Bottle 1: x 2 Buffer (12 mL, pH 7.0) plus p-hydroxybenzoic
acid and sodium azide (0.02% w/v) as a
preservative.
Stable for > 5 years at -20°C.
Bottle 2: x 2 Peroxidase plus 4-aminoantipyrine and stabilisers;
lyophilised powder.
Stable for > 5 years at -20°C.
Bottle 3: D-Glucose (~ 10 g).
Stable for > 5 years stored dry at room temperature.
Bottle 4: Glucose oxidase standard (approx. 2.9 U; actual
value stated on the vial label).
Stable for > 5 years at -20°C.
Bottle 5: Buffer (5 mL, pH 7.0) plus BSA (0.5% w/v) and
sodium azide (0.04% w/v) as a preservative.
Stable for > 2 years at 4°C.