A simple, reliable, rapid and accurate method for the measurement of glycerol in beverages, foodstuffs and other materials.
INTRODUCTION:
In the food industry, glycerol is an important moistening agent
for baked goods. It is also added to candies and icings to prevent
crystallisation and as a solvent for food colours and carrier for
extracts and flavouring agents. As a product of fermentation, glycerol
is monitored in the beer and wine industries, where it occurs at
concentrations of approx. 1% (v/v) and is an indicator of quality.
The smoothness of lotions, creams and toothpaste is due to the
incorporation of glycerol. Due to its humectant properties, glycerol
is sprayed on pre-processed tobacco to prevent crumbling.
This kit benefits from the presence of NADH, ATP and PEP in the
form of highly stable tablets.
KITS:
Kits suitable for performing 70 assays in manual format (or 700 assays
in microplate format) are available from Megazyme. The kits contain
the full assay method plus:
Bottle 1: Buffer (20 mL, pH 7.4) plus sodium azide
(0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: Tablets (fourteen) containing NADH plus ATP and
PEP. Stable for > 2 years at 4°C or -20°C.
NOTE: Maximum long-term stability of the tablet components is
obtained by storage of the tablet bottle in a sealed container in the
presence of a drying agent such as silica gel at 4°C or at -20°C.
Bottle 3: Pyruvate kinase plus L-lactate dehydrogenase
suspension, 1.5 mL.
Stable for > 2 years at 4°C.
Bottle 4: Glycerokinase suspension (1.5 mL).
Stable for > 2 years at 4°C.
Bottle 5: Glycerol standard solution (5 mL, 0.20 mg/mL)
in 0.02% (w/v) sodium azide.
Stable for > 2 years at 4°C.