L-海藻糖检测试剂盒 L-Fucose Assay Kit

产品货号：K-FUCOSE

品牌：Megazyme

产品品名：L-海藻糖检测试剂盒

英文品名：L-Fucose Assay Kit

规格型号：100 assays (manual) / 1000 assays (microplate) / 1020 assays (auto-analyser)

A simple, rapid and reliable method, for the measurement of L-Fucose in plant extracts, biological samples and other materials. This kit can be used in the measurement of α-fucosidases that do not act on chromogenic substrates.


INTRODUCTION:

L-Fucose is a 6-carbon deoxyhexose and exists in nature in various biological niches. A major natural source of L-fucose is the brown algal polysaccharide fucoidan.
L-Fucose is a minor component in plant cell wall polysaccharides, specifically, rhamnogalacturonan, xyloglucan and also arabinogalactan proteins that are involved in plant cell elongation.
In glycoproteins L-fucose is a key component of the oligosaccharide moiety and is attached via α-1,(2, 3, 4 or 6) linkages. The variation of the oligosaccharide structure is known to dictate biological function of glycoproteins and, as such, L-fucose has been indicated as a potential biological marker for various disease states such as breast cancer. L-fucose is a common component of human milk oligosaccharides (HMO) such as 2’-fucosyl-lactose. In addition to the role as a prebiotic, HMOs are known to impart immunological functions to breast-fed infants such as preventing microbial adhesion to the intestinal mucosal membrane.
This kit (K-FUCOSE) provides a rapid and reliable method, suitable for the measurement of L-fucose in plant extracts, biological samples and other materials. It can also be used in the measurement of α-L-fucosidases that do not act on chromogenic substrates.

KITS:

Kits suitable for performing 100 assays in manual format (or 1000 assays in microplate format or 1020 assays in auto-analyser format) are available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (44 mL; pH 9.5) plus sodium azide
(0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: NADP+.
Freeze dried powder.
Stable for > 5 years at -20°C.
Bottle 3: L-Fucose dehydrogenase suspension (2.2 mL).
Stable for > 2 years at 4°C.
Bottle 4: L-Fucose (5 mL, 0.5 mg/mL).
Stable for > 2 years at 4°C.

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