A rapid, reliable and accurate method for the specific measurement of D-fructose and D-glucose in wine, beverages, foodstuffs and other materials. Supplied as a "ready to use" liquid stable formulation that is suitable for autoanalyser and microplate formats.
INTRODUCTION:
D-Glucose and D-fructose are found in most plant products. In foods, they are present in significant quantities in honey, wine and beer, and a range of solid foodstuffs such as bread and pastries, chocolate and candies. In the wine industry, the sum of D-glucose and D-fructose, termed “total residual sugars”, is a key parameter, as this represents the amount of sugar that is available to the yeast for the conversion into ethanol. Total residual sugar levels are monitored throughout the fermentation and after fermentation is complete are adjusted to achieve the desired taste profile. For the vast majority of measurements taken during the wine making process it is unnecessary to differentiate between the D-glucose and D-fructose, allowing them to be quantified simultaneously.
This kit (K-FRGLQR) is suitable for the specific measurement of D-glucose and D-fructose in wines, beverages, foodstuffs and other materials. The procedure formats provided in this booklet allow for the individual or simultaneous measurement of D-glucose and D-fructose.
KITS:
Kits suitable for performing 1100 assays of each test in auto-analyser format are available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Reagent 1 (44 mL)
Contains sodium azide (0.08% w/v) as a preservative. Ready to use.
Stable for > 2 years at 4°C.
Bottle 2: Reagent 2 (22 mL)
Contains sodium azide (0.05% w/v) as a preservative. Ready to use.
Stable for > 2 years at 4°C.
Bottle 3: Reagent 3 (22 mL)
Contains sodium azide (0.05% w/v) as a preservative. Ready to use.
Stable for > 2 years at 4°C.
Bottle 4: D-Glucose plus D-Fructose Standard (2 mL)
(3 g/L of each sugar). Ready to use.
Stable for > 2 years at room temperature.