乙酸【AK法】检测试剂盒 ACETIC ACID(ACETA
TE KINASE FORMAT) assay Kit 产品货号：K-ACETAK 规格型号：550次（170.5 ml）- 询价
- Megazyme
- K-ACETAK
- 2025年07月09日
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产品货号：K-ACETAK
品牌：Megazyme
产品品名：乙酸【AK法】检测试剂盒
英文品名：ACETIC ACID(ACETATE KINASE FORMAT) assay Kit
规格型号：550次（170.5 ml）
Analyser format for the specific assay of acetic acid (acetate) in beverages and food products. On calibration, the prepared reagent is linear to > 28 micrograms of acetic acid per mL of assay solution.
INTRODUCTION:
The most widely used method for enzymatic quantification of acetic
acid is that employing acetyl-coenzyme A synthetase (ACS). However,
this method is based on the use of an indicator reaction catalysed by
L-malate dehydrogenase that is in permanent equilibrium, and therefore
a non-stoichiometric increase in absorbance is observed from the
acetate present in the sample. Thus, a slightly non-linear response
to increasing acetate concentration is observed upon calibration,
resulting in poor R2 values. In addition (depending on the supplier)
reagents prepared for auto-analyser applications can have very limited
on-machine stability, due to rapidly increasing blank absorbance values.
To overcome these issues, Megazyme developed this alternative acetic
acid kit, based on the enzyme acetate kinase (AK; see equations 1-3
below), especially for the auto-analyser user. This reagent has improved
on-machine stability, gives excellent linear calibration curves, and results
in a stoichiometric change in absorbance due to the acetic acid present
in the sample.
KITS:
Kits suitable for performing a minimum of 550 assays in auto-analyser
format are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: Buffer (11 mL, pH 7.4) plus sodium azide (0.02% w/v ) as a
preservative. Stable for > 2 years at 4°C.
Bottle 2: NADH plus ATP, PEP and PVP.
Stable for > 2 years at either 4°C or -20°C.
Bottle 3: Acetate kinase plus pyruvate kinase and D-lactate
dehydrogenase suspension, 4.1 mL.
Stable for > 2 years at 4°C.
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Solutions 1.1 M Acetic Acid 2.Buffer A Homogenization 10 mM MOPS- 40 ml of 2M stock solution 8 liters 4 mM EDTA- 160ml of 0.2M stock solution 1 mM DTT* - 8ml of 1M stock solution 0.1 mM PMSF* - 14ml of 10mg/ml stock solution 10 μg/ml trypsin
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