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文献和实验RNA Purification Protocol for 100-200 mg of Animal Tissue
设备 1. Centrifuge capable of 3,000-5,000 x g 2. High speed centrifuge tube with capacity of at least 15 ml 3. Ice bath 4. Ground pestle 实验步骤 1. Dissect tissue quickly and freeze in liquid nitrogen
into smaller pellets and processed more easily in their tubes. 4). Wash pellets with 200 mM sodium cacodylate buffer (3 x 5'). At this stage, the samples will be fixed so can be washed in any sutable buffer, and even water. Sodium cacodylate is suggested
Immunofluorescence on ultrathin resin sections
General remarks: Ultrathin methacrylate-(e.g.Lowicryl HM20, K4M, Monostep polar and nonpolar or LR-White) or epoxy (Epon/Araldite, Spurr) sections are transferred with a loop on poly-L-lysine coated, round cover slips
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