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文献和实验TR Tn3 terminal inverted repeats lacZ 5'-truncated lacZ gene encoding beta-galactosidase LEU2 LEU2 gene from S. cerevisiae amp Encodes beta-lactamase loxP lox site, target for Cre
Methods for use with the mTn-lacZ/LEU2-mutagenized library
. It is then transformed into a leu2 strain of yeast. This procedure is outlined in this figure . Use of a circle-zero strain will prevent recovery of insertions in the 2-micron plasmid. The best strategy is to screen a few thousand transformants from each pool
Replication timing by comparative hybridization
the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml
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