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文献和实验Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)
. Add 10ml SPM Buffer to the sample. 14. Resuspend the Mag-Bind particles pellet by vortexing. Incubate 3 minutes at room temperature. Complete resuspension of the Mag- Bind particles pellet is critical to obtain good results. 15. Place
Mag-Bind Blood DNA Maximum Yield Protocol (7ml-10ml)
separation device. Add 5 ml Buffer MP/Ethanol Mixture to the sample. Note: MP/Ethanol mix has to be prepared freshly. 9. Resuspend the Mag-Bind particles pellet by vortexing. Incubate 3 minutes at room temperature. During incubation, mix
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
to get through these as quickly as possible. 1. Spin down beads @10K, 1min, RT. 2. Aspirate supernatant. 3. Wash with 1ml of the following buffers and mix by inverting 3 or 4 times. Only aspirate supernatant to 0.1ml marking so no beads are lost: o FA lysis buffer
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