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文献和实验licanming 我的实验是用VEGF处理细胞后测定细胞PKC激活水平，拟采用Western blot的方法，用ECL法检测，这样分析PKC激活程度结果国际上认可吗? 我在Cell Signaling Technology上，查到 2261 Phospho-(Ser) PKC Substrate Antibody W IP E D 9371 Phospho-PKC (pan) (betaII Ser660) Antibody W IP
推荐一本书－－Protein Arrays: Methods and Protocols
and construct protein arrays for basic and clinical research in a high-throughput manner. These readily reproducible techniques can be applied to such applications as quantifying specific proteins of interest and discovering novel proteins
Intracellular Cytokine Staining Protocol
of interest (see specific instructions). Stain cell-surface antigen following the Surface Staining Protocol. The choice of the surface marker depends on the experimental question. For suggestions on best phenotypic markers for different cell types
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