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文献和实验Assaying Cdc25 Phosphatase Activity
To determine the activity of all human Cdc25 phosphatases, two different methods are described. For assaying phosphatase activities of recombinant Cdc25 proteins produced in Escherichia coli or insect cells, a fluorimetric assay using
Analysis of MicroRNA Length Variety Generated by Recombinant Human Dicer
blotting and employed this method to investigate cleavages generated by recombinant human Dicer in the synthetic miRNA precursors. We paid special care to visualize clearly the cleavages generated by the individual RNase III domains of Dicer
. Fluorometric assays incorporating recombinant human enzymes are ideally suited for this purpose.
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