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文献和实验Transformation of E. coli by Electroporation
100% ethanol 70% ethanol 0.5X TE SOC medium transformation plates I. Preparation of E. coli cells
Analysis of total E. coli protein by SDS PAGE
microliters to Novex NuPAGE gels and stain with commassie blue. Note: Boiled cells can be viscous due to the cellular DNA. If very viscous (hard to load on gel), try to pipet the SDS PAGE loading buffer from a region of the tube with a low viscosity. 1 X SDS
Strategies to Optimize Protein Expression in E. coli
in Escherichia coli (E. coli ) is simple, fast, inexpensive, and robust, with the expressed protein comprising up to 50 percent of the total cellular protein. However, it also has disadvantages. For example, the rapidity of bacterial protein expression
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