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文献和实验Recombinant Single Globin-Chain Expression and Purification
with methionine aminopeptidase (MAP) cDNA.
Expression of Recombinant Proteins with Uniform N-Termini
to a self-splicing mini-intein. This fusion construct is expressed in an engineered E. coli strain from which the pepP gene coding for aminopeptidase P has been deleted. We describe a protocol using human cationic trypsinogen as an example to demonstrate
Assays for Human DNase I Activity in Biological Matrices
Pulmozyme� recombinant human deoxyribonuclease I (DNase I) is currently used as a therapeutic for cystic fibrosis (CF) (1 ) and may be effective in the treatment of systemic lupus erythematosus (SLE) (2 ,3 ). As described in Chapter 20
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