PolyFect Transfection Reagent

TaKaRa RNAiso Reagent

使用，不要用于其它实验。【试剂配制】用于RNA实验的试剂，须使用干热灭菌（180℃，60分钟)或使用上述方法进行DEPC水处理灭菌后的玻璃容器盛装(也可以使用RNA实验用的一次性塑料容器)，使用的无菌水须用0.1%的DEPC处理后再进行高温高压灭菌。RNA实验用的试剂和无菌水都应专用，避免混用后交叉污染。2. 实验样品的研磨和匀浆。 A. 贴壁培养细胞 ① 倒出培养液，用1×PBS清洗一次。 ② 每10 cm2 生长的培养细胞中加入2 ml的RNAiso Reagent，水平放置片刻

Cationic Liposome-Mediated Transfection with Lipofectin Reagent

Since their original description, liposomes have been discussed as vehicles that could be used as carriers of pharmaceutically active agents (1 ), and their potential for use as carriers of genetic information has been examined

PCR Reagent System

isolated from the other components. PCR products should be analyzed in an area separate from the reaction assembly area.  1) Add the following components to a sterile 0.5-mL microcentrifuge tube on ice:  2) Cap the tubes and centrifuge briefly