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- 保存条件：
-20°c
- 库存：
1
- 英文名：
T7SELECTUP PRIMER
- 供应商：
Merck
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文献和实验Primer Design（Manual and Automated Primer Design for SEQ and PHY Gaps）
General Primer General Primer Design Guidelines Note the 5’-3’ direction of the contig. Locate primers 100 to 200 bp from the feature. Pick primer from ≥ 2x high quality sequence coverage
in vitro8) Anneal oligo A and oligo B separately to T7 primer (TAATACGACTCACTATAGG) or toprimers that are complementary to oligoA and B.9) Use 2-3 ug annealed primer in a 20ul Ambion T7 Megashort script reaction Ambion Cat# 1354 (Follow Ambion’s protocol
Optimal arbitrary primer length for Differential Display（图）
rather than scientific reasons. The initial idea was to use one-base as an anchor, but the cloned murine thymidine kinase (TK) cDNA originally used as a control cDNA template had only 11 A's in its poly(A) tail. It was found that one-based anchored primer T7C up to T
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