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- 英文名:
Puregene Blood Core Kit C
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文献和实验E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
tubes or microplates 实验步骤 1. Add 1-100 ul blood sample to a nuclease-free microcentrifuge tube. Bring the volume up to 100 ul with Elution Buffer provided with this kit. 2. Add 100 ul Buffer MSL and 10 ul Proteinase K
Isolation of PBMCs from patient blood and buffy coats
can be stored in liquid nitrogen for more than 10 years. Determination of a 32 bp deletion in the CCR5 gene of PBMCs donors 7| Isolate genomic DNA from 1 106 PBMCs or 200 ml of blood using the Qiagen blood kit or equivalent alternative
A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
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