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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
552.2 Lab Performance Check Solution, Various Conc.
- 库存:
1
- 供应商:
甄准生物
- 规格:
25 ml
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文献和实验Marcantonio Lab Protocol Manual
, top off with CsCl/TE solution containing 0.2 mg/ml of EtBr, and repeat steps 3,4, and 5. 7) Remove the ethidium bromide by extraction using an equal volume of TE-saturated n-butanol. Vortex the tube vigorously and remove the upper organic phase. Repeat
Northern Blotting方法(Howell Lab)
water (check weight to account for evaporation) Add 20 ml 10X MOPS and let cool on stir plate to about 56℃. Add 36 ml 37% formaldehyde. Pour into Hoefer appartatus when gel solution is below 50℃. Running buffer: dilute 100 ml 10
To ddH2O, add DEPC to 0.1% Shake to get the DEPC droplets into solution Leave at 37deg. overnight Autoclave 20 minutes to destroy the DEPC 0.1 M sodium phosphate pH 7.0 (make 1 liter) Adjust to the appropriate pH by mixing
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