AssayPro—Mouse Leptin ELISA Ki

Introduction
Leptin, a 16-kDa protein secreted from white adipocytes, has been implicated in the regulation of food intake, energy expenditure, and whole-body energy balance in rodents and humans (1). Leptin has been a potential target for treating obesity. The plasma insulin response appears more closely associated with the plasma leptin concentration (2). Neonatal leptin levels are strongly associated with female gender, birth length, and formula feeding (3). Leptin concentrations were higher in women than in men. In women, serum leptin was the most important predictor of myocardial infarction (MI) (4). In patients with angiographically confirmed coronary atherosclerosis leptin is a novel predictor of future cardiovascular events independent of other risk factors, including lipid status and CRP (5). Leptin may also play an important role in the pathophysiology of osteoarthritis (OA) (6).

Principle of the Assay
The AssayMax Mouse Leptin ELISA kit is designed for detection of mouse leptin in plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures leptin in less than 5 hours. A polyclonal antibody specific for leptin has been pre-coated onto a microplate. Leptin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for leptin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.