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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
AssayPro
- 检测范围:
1 ng/ml
- 检测方法:
ELISA
- 应用:
血清、血浆、细胞培养上清液、尿液
- 样本:
50 μl
- 规格:
96 wells
小鼠C反应蛋白酶联免疫试剂盒
Introduction
C-Reactive Protein (CRP) is a liver protein composed of five identicalnon-glycosylated subunits, with a total molecular weight of 105 kDa. CRPhas a variety of powerful effects related to immunology, inflammation,and coagulation. As a marker of low-level inflammation, CRP appears topredict future cardiovascular disease events among apparently healthyindividuals. High plasma concentration of CRP was associated withincreased risk of stroke, myocardial infarction, and peripheral vasculardisease (1, 2, 3). CRP has also been associated with increased risks offatal coronary events among high-risk male smokers and incident coronarydisease among the elderly (4, 5). Studies have established theprognostic usefulness of CRP in the setting of angina (6). Originallyused as a marker of acute inflammation, CRP has become a leadingcandidate as the measure of choice for estimating the inflammatorycomponent of cardiovascular disease risk.
Principle of the Assay
The AssayMax Mouse C-Reactive Protein ELISA kit is designed for detectionof Mouse CRP in urine, plasma, serum, and cell culture supernatants. Thisassay employs a quantitative sandwich enzyme immunoassay technique thatmeasures CRP in less than 4 hours. A polyclonal antibody specific forrat CRP has been pre-coated onto a microplate. CRP in standards andsamples is sandwiched by the immobilized antibody and biotinylatedpolyclonal antibody specific for CRP, which is recognized by astreptavidin-peroxidase conjugate. All unbound material is then washedaway and a peroxidase enzyme substrate is added. The color developmentis stopped and the intensity of the color is measured.
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文献和实验人 C- 反应蛋白 (CRP) 酶联免疫分析(ELISA) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中 C- 反应蛋白(CRP) 的含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中 人 C- 反应蛋白 (CRP) 水平。用纯化的 人 C- 反应蛋白 (CRP) 抗体 包被微孔板,制成固相抗体,往包被单抗的微孔中加入 C- 反应蛋白 (CRP) ,再与 HRP 标记的 C
人 高敏 C 反应蛋白( hs-CRP ) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中 高敏C 反应蛋白(hs-CRP ) 的 含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中 人高敏 C 反应蛋白( hs-CRP ) 水平。用纯化的 人 高敏 C 反应蛋白( hs-CRP ) 抗体包被微孔板,制成固相抗体,往包
Laboratory Assessment of the Acute Phase Response: Using CRP as a Model
by the relative insensitivity of the assays then available; we simply could not detect low levels of the markers of inflammation. In the last few years, however, C-reactive protein (CRP) assays of increasing sensitivity have shown that, in fact, the continuum
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