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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
HCl Assay Reagent (20 mM)
- 库存：
1
- 供应商：
甄准生物
- 保存条件：
-20.0
- 规格：
1 ml
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文献和实验Extraction of DNA using DNAzol Reagent
1 ml of DNAzol® Reagent to 1-3 × 107 cells, either in pellet or in suspension (volume 100 µl), pellet the cells and wash them with 0.9% NaCl. Pellet the cells again and resuspend them in one volume of cold (4°C) hypotonic solution [20 mM Tris HCl (pH
: 55° C for 30 s c. Extend: 72° C for 1 min 30 s 5) Incubate for an additional 10 min at 72° C and maintain the reaction at 4° C. The samples can be stored at –20° C until use. 6) Analyze the amplification products by agarose gel
Clonality - X Chromosome Inactivation Assay
can be utilized. 1. Materials DNA sample (see Processing of Microdissected Tissue - DNA-based Analysis) Proteinase K (Sigma) Proteinase K buffer (0.05 M Tris-HCL, 0.001 M EDTA, 1% Tween 20, 0.1 mg/ml proteinase K, pH 8.0) Phenol:chloroform:isoamyl alcohol
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