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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
HeLa acid extract
- 库存：
1
- 供应商：
甄准生物
- 保存条件：
Dry Ice
- 规格：
100 µg
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文献和实验Largescale nuclear extract preparation
,Brenner D (1990)A simplified method for the preparation of transcrip-tionally active liver nuclear extracts.DNA Cell Biol.Vol.10,P.777-781 I.Homogenize cells or tissue 1.Wash commercially available HeLa cells (30g)or minced in 3mm pieces
Large scale nuclear extract preparation
or tissue 1.Wash commercially available HeLa cells (30g)or minced in 3mm pieces fresh liver(15-20g)from staining with homogenizing buffer (10ml)and transfer it to 100ml beaker 2.Add to the beaker another 25ml of the buffer and split the content in half (2x
Large scale nuclear extract preparat
1.Wash commercially available HeLa cells (30g)or minced in 3mm pieces fresh liver(15-20g)from staining with homogenizing buffer (10ml)and transfer it to 100ml beaker 2.Add to the beaker another 25ml of the buffer and split the content in half (2x15
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