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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
6β-Μethyl Prednisolone 21-Acetate
- 库存：
1
- 供应商：
甄准生物
- CAS号：
1048031-82-8
- 规格：
25mg /
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文献和实验Thin-Layer Chromatography Analysis of Human CYP3A-Catalyzed Testosterone 6-Hydroxylation
microsomes. 14 C-labeled enzymatic products are applied to silica gel thin-layer plates, which are developed sequentially with methylene chloride:acetone (80:20) followed by chloroform, ethyl acetate, and absolute ethanol (80:20:14). Metabolite quantification
Transformation of E. coli by Electroporation
ionic strength and a high resistance. The DNA may be purified by either precipitation or dialysis. Purifying DNA by Precipitation: 1. Add 5 to 10 μg of tRNA to a 20 μl ligation reaction. Add 22 μl 5M ammonium acetate. Mix well. 2. Add 100 μl
Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry
, the enzymatic reactions are quenched using an ethyl acetate/methanol solution and all five enzyme solutions are combined. The mixtures of the reaction products are prepared using liquid–liquid and solid-phase extractions and quantified simultaneously using
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