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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Deca-2,4,6,8-tetraenal
- 库存:
1
- 供应商:
甄准生物
- CAS号:
40650-87-1
- 保存条件:
Amber Vial, -86˚C Freezer
- 规格:
50mg / 500mg
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文献和实验., PartiSphere SCX, Whatman) Tandem mass spectrometer A variety of mass spectrometers are suitable, including the LCQ Classic, Deca, Duo, or TSQ series (ThermoFinnigan), or the QTOF1 or QTOF2 (Micromass, Inc.). ThermoFinnigan LCQ Xcalibur software
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
0.1% TFA Inject ~ 5 µl in the LCQ Deca from Finnigan 2. Reduction and alkylation Wash the particles with 100-150 µl of water (5min), spin down and remove the liquid. Add acetonitrile (3-4 gel volumes) and wait for 10-15 min
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
solution'' Freeze in liquid nitrogen, then dry in a Speed-vac. At this point, samples can be stored at -20°C. 6. Mass spectrometry Dissolve the sample in ~ 10 µl 0.1% TFA Inject ~ 5 µl in the LCQ Deca from Finnigan
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