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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Cyanidin 3-O-β-D-Galactopyranoside Chloride
- 库存：
1
- 供应商：
甄准生物
- CAS号：
27661-36-5
- 保存条件：
Amber Vial, -20˚C Freezer, Under Inert Atmosphere
- 规格：
1mg / 10mg
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文献和实验Measurement of Chloride Movement in Neuronal Preparations
(GABA) or other agonist in recipeRingers solution Plexiglas imaging chamber (Fig. ) with outflow and inflow tubes (∼1.7‐mm‐o.d.; ∼1.2‐mm i.d.), flow rate restrictor (in line
reached 1.0, isopropyl-1-thio-β-d-galactopyranoside was added to a final concentration of 1 mM. After 5 h of induction, the cells were harvested by centrifugation at 4,000 × g for 20 min. 样品处理 For the purification of protein from 2 liters of culture
Preparation of Competent Cells (from PMB)
1.Inoculate a couple of 20 ml LB broth in a 125 ml flasks with 0.5 ml of an overnight culture of the recipient strain. 2.Shake at 37 ℃ until O.D. 600=0.13 - 0.15 (1 - 2 hours). Measure by taking some of the broth out with a sterile DPTP
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