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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
6-Carboxytetramethyl Rhodamine, 90%
- 库存：
1
- 供应商：
甄准生物
- CAS号：
91809-67-5
- 保存条件：
-20°C Freezer
- 规格：
10mg / 100mg
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文献和实验Preparation of Segmented and Polarity Marked Microtubules
consisting of 15 µM tubulin (1 part rhodamine tubulin to 10 parts unlabeled tubulin ) and 12 µM NEM GTP-tubulin in 1X BRB80, 1 mM DTT, 1 mM GTP. Incubate at 0¡C for 5' and (optionally) clarify at 90K for 5' at 2¡C in a TLA100 rotor. 3. Incubate dim polar
Preparation of Segmented and Polarity Marked Microtubules
) 1:9 rhodamine labeled : unlabeled tubulin and 8 µM NEM CPP-Tubulin in 1X BRB80, 1 mM DTT, 0.5 mM GMPCPP. Incubate on ice for 5'-10', spin 90K 5' in TLA100 at 2¡C, freeze in liquid nitrogen in 10 µl aliquots (or use fresh). 2. Thaw GMPCPP bright seed
New Rapid Multicolor PRINS Protocol
. With this protocol, one can create a third color using the two most common forms of labeled dUTP (biotin- and digoxigenin-labeled dUTP) and two fluorochromes (fluorescein and rhodamine). The signals at the centromeres of three different chromosomes displayed perfect
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