LD Biopharma Recombinant Human

Recombinant Human Oct4_V16-11R Protein，品牌LD Biopharma，货号HTF-0279，规格50 µg / Vial。 基因符号：Oct4_VP16 登录号：NP_002692.2 and NP_044650.1 物种：Human 质量控制：1. Purity: > 90% by SDS-PAGE. 2. DNA binding assay: DNA binding activity was demonstrated using Oct4 specific DNA binding oligo derived EMSA. 制剂组成：1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. 英文说明：Human Oct4 protein encoded by POU5F1 gene is a homeodomain transcription factor of the POU family. This protein is critically involved in the self-renewal of undifferentiated embryonic stem cells. As such, it is frequently used as a marker for undifferentiated cells. Oct4 combined with Sox2, Klf4 and cMyc has been used for generating iPS cells. To further increase Oct4 activity for gene transcription activation, HSV VP16 TAD (transcription activation domain, 76aa) was fused with human Oct4 protein with unique linker domain: L5m. Recombinant human Oct4_VP16 TAD gene was fully synthesized with codon optimization for E. coli expression. It was constructed with C-terminal tag of 11 arginine domain, which efficiently delivery protein intracellularly. This protein was expressed in E. coli as inclusion bodies, refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified. Incubating this protein in various culture mediums at concentration of 2-8 µg/ml may be used for protein derived iPS generation when combined with Sox2, Klf4 and cMyc. 应用信息：1. Protein transduction for protein derived iPS generation. 2. Active recombinant protein, may be used for ELISA based DNA/Protein binding assay. 3. As specific protein substrate for kinase assay. 本品仅供科研使用。实际构建、质量控制、保存及使用要求请以批次文件为准；网站目录不代表当前库存或实际供货承诺。