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- 文献和实验
- 技术资料
- 库存:
999
- 英文名:
Human CB1 / CNR1 CHO Cell Line
- CAS号:
NONE10717
- 供应商:
上海麦克林生化科技股份有限公司
- 保存条件:
-80℃
- 规格:
5E6cells/EA
商品名称: Human CB1 / CNR1 CHO Cell Line
商品中文名称: Human CB1 / CNR1 CHO Cell Line
商品英文名称: Human CB1 / CNR1 CHO Cell Line
商品CAS: NONE10717
商品型号: H997642
相关规格: 5E6cells/EA
对应价格: 28000
商品货期: 预计交期:8-10天
商品纯度: 无
商品分子式: 无
商品分子量: 无
储存条件: -80℃
运输条件: 干冰

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文献和实验cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Passages: 17 Karyotype: Hypodiploid, modal No 20Hazard: CYA subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline
Establishment of Stable Transfectant of CHO Lec Cells
Cells should be 50~70% confluent. Detach cells with trypsin/EDTA, wash once w/ complete medium, and count cell number.Suspend cells in EPBS at ~1 x 107/ml (Typical yield is ~2 x 107 cells/flask)Add 800 µl of cells to cuvette plus DNA (dissolved
of antibodies, which might negatively effect cell growth.Our first procedure will involve the simple transfer of an established culture from a suspension culture. An aliquot will be removed from a commercially available cell line and transferred to prepared
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