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- 文献和实验
- 技术资料
- 英文名:
Recombinant Human Alkaline Phosphatase (Germ type)/ALPG Protein,mFc Tag
- 保存条件:
-25 ~ -15℃保存,收到货之后有效期1年。 复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
- 规格:
25 μg(95057ES25)/100 μg(95057ES60)/500 μg(95057ES76)
| 规格: | 25 μg(95057ES25) | 产品价格: | ¥1215 |
|---|---|---|---|
| 规格: | 100 μg(95057ES60) | 产品价格: | ¥4445 |
| 规格: | 500 μg(95057ES76) | 产品价格: | ¥14855 |
产品属性:
| 产品别称 | Alkaline phosphatase, placental-like,PLAP-like,ALP-1,Alkaline Phosphatase |
| 种属 | Human |
| 表达序列 | Ile20-Asp503 |
| Accession | P10696 |
| 标签 | C-mFc |
| 表达系统 | HEK293 |
| 分子量 | The protein has a predicted MW of 78.24 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on SDS-PAGE result. |
| 纯度 | > 95% as determined by SDS-PAGE and HPLC. |
| 内毒素 | < 1.0 EU per μg by the LAL method. |
| 制剂 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 外观 | Lyophilized Powder |
| 生物活性 | 【ELISA Data】 Immobilized Human Alkaline Phosphatase (Germ type) , mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 29.3ng/ml determined by ELISA. |
| 复溶方法 | 开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。 |
产品特色:
高纯度、高活性、低内毒素、高批间一致性
储存条件:
-25 ~ -15℃保存,收到货之后有效期1年。 复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
salt NBT, (Altman, 1972) can be used in conjunction with X-Phos as the final electron acceptor for the indoxyl dimerization reaction. When reduced, NBT forms a purple precipitate. Of the cloned phosphatases, the human placental alkaline
Genetic and Pharmacologic Models for Type 1 Diabetes
. , Serreze, D.V. , and Chen, Y.G. 2011. Mouse models for the study of autoimmune type 1 diabetes: A NOD to similarities and differences to human disease. Semin. Immunopathol. 33: 67‐ 87.
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
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