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- 文献和实验
- 技术资料
- 英文名:
Recombinant Biotinylated Human IL-23 alpha&IL-12 beta Protein,His-Avi Tag
- 保存条件:
-25 ~ -15℃保存,收到货之后有效期1年。 复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
- 规格:
100 μg(94323ES60)/500 μg(94323ES76)/25 μg(94323ES25)
| 规格: | 100 μg(94323ES60) | 产品价格: | ¥9275 |
|---|---|---|---|
| 规格: | 500 μg(94323ES76) | 产品价格: | ¥28855 |
| 规格: | 25 μg(94323ES25) | 产品价格: | ¥2555 |
产品属性:
| 产品别称 | IL23 alpha, IL12 beta, IL23 alpha&IL12 beta , IL12 β, IL23 alpha&IL12 β, IL-23A&IL-12B, IL23A&IL12B |
| 种属 | Human |
| 表达序列 | Arg20-Pro189(IL-23 alpha)&Ile23-Ser328(IL-12 beta) |
| Accession | Q₉NPF₇(IL-23 alpha)&P29460(IL-12 beta) |
| 标签 | C-His-Avi |
| 表达系统 | HEK293 |
| 分子量 | The protein has a predicted MW of 21.7 (IL-23 alpha)&34.7 (IL-12 beta) kDa. Due to glycosylation,the protein migrates to 24 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) kDa based on Tris-Bis PAGE result. |
| 纯度 | > 95% as determined by SDS-PAGE. |
| 内毒素 | < 1.0 EU per μg by the LAL method. |
| 制剂 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 外观 | Lyophilized Powder |
| 生物活性 | 【ELISA Data】Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. |
| 复溶方法 | 开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。 |
产品特色:
高纯度、高活性、低内毒素、高批间一致性
储存条件:
-25 ~ -15℃保存,收到货之后有效期1年。 复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
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文献和实验Western Blotting with Biotinylated Antibodies
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
Human IL-2 Enzyme Immunoassay (EIA)
Increased division and maturation of activated helper T cells Stimulation of neutrophils Stimulus for the secretion of interferon-( and tumor necrosis factors - aphla and - Beta from peripheral mononuclear cells. The Endogen Human IL-
Western Blotting with Biotinylated Antibodies
Western Blotting with Biotinylated Antibodies SAMPLE PREPARATION For Protein Concentration Determination of Cell Culture Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline
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