SuperMag Streptavidin Beads, 1

Chromatin IP (CHIP assay)

and measure protein concentration (BioRad assay) use 1 - 5 mg protein per IP immunoprecipitate for 2 h to ON, 4 °C wash immunoprecipitations pelleting the beads each time:      2 x 1 ml CHIP lysis buffer      2 x 1 ml CHIP

MACS(磁珠分选)技术相关产品 （dynal公司）

.16 112.05 Dynabeads M-280 Streptavidin Supplied at 6.7x108 beads per ml (10mg/ml) in PBS, pH7.4 with 0.1% BSA and 0.02% sodium azide. Isolation of any biotinylated target. Applications include DNA, RNA and protein isolation

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

   3.75ml 4M NaCl       3ml 10% Triton X (Tx-100)       300µl 10% NAD       21.45ml H20 ChIP wash buffer       30ml final volume:       150µl 2M Tris       1.7ml 4.4M LiCl       60µl 0.5M EDTA       1.5ml 10% NAD       1.5ml 10% NP-40       25.09ml