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文献和实验) A431 cells. At 48 h after infection, these cells were stained with ten different primary antibodies (5 g/ml) specific for different cell surface markers: anti-CD15 (clone 2F3; BD, PharMingen, San Diego, CA), anti-ERBB-2 (clone SP77; ref. 5), anti-ERBB
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
al. 2006b ). MATERIALS Reagents Anti-CD24 monoclonal antibody (mAb) (J11d clone) (for Protocol 3) Use either culture supernatant that contains anti-CD24 mAb or purified anti-CD24 mAb (see Step 55). BDPharmLyse (red blood cell lysing
Pyrocarbonate (DEPC) treated H2O Master set of clone-purified, sequence verified human ESTs (e.g. gf211 release, Research Genetics, Huntsville, AL) 96 pin inoculating block (#VP 4088, V&P Scientific, Inc, San Diego, CA) Airpore Tape Sheets, (# 19571
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