Pig Whole Blood Non-Sterile wi

Isolation of Non-muscle Actin

for isolation of Erythrocyte Actin 1. Collect blood from animal using 20 ml/100 ml whole blood acid citrate/dextrose (0.085 M sodium citrate, 0.065 M citric acid 2% dextrose) as anti-coagulate. 2. Centrifuge blood at for 10 min at 4300 x g and remove

Flow cytometric determination of leukocyte surface antigens in whole blood

by standard venipuncture into an appropriate anticoagulant. Use EDTA or heparin (10 iu/ml). Do not use citrate, this produces an acidic environment which will quench your fluorescein labeled antibody! Collect blood with a large gauge needle into a syringe

染色体组型分析（Karyotype Analysis）

, prepared fresh)      Dry ice      Slides, coverslips and permount      Alkaline solution for G-banding      Saline-Citrate for G-banding      Ethanol (70% and 95% (v/v))      Giemsa stain Procedure Use Table Draw 5 ml of venous blood into a sterile