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- 文献和实验
- 技术资料
- 供应商:
MyBioSource
- 检测范围:
2-600ng/mL
- 检测方法:
Quantitative Sandwich
- 适应物种:
Monkey
- 样本:
Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids
- 灵敏度:
0.91ng/mL
- 规格:
48-Strip-Wells
MyBioSource Monkey Complement 4, C4 ELISA Kit|MBS1604166-48T|Monkey
【产品详情】
MyBioSource MBS1604166-48T(Monkey Complement 4, C4 ELISA Kit)为 Monkey 检测试剂盒,采用 Quantitative Sandwich 方法,适用于 Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids 样本中 Complement 4 的定量检测。
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Monkey C4 antibody. C4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Monkey C4 Antibody is added and binds to C4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated C4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Monkey C4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm. Intended Uses: This sandwich kit is for the accurate quantitative detection of Monkey Complement C4 (also known as C4) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
【产品参数】
| 项目 | 信息 |
|---|---|
| 品牌 | MyBioSource |
| 货号 | MBS1604166-48T |
| 产品名称 | Monkey Complement 4, C4 ELISA Kit |
| 检测对象 | Complement 4 |
| 种属 | Monkey |
| 样本类型 | Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids |
| 检测方法 | Quantitative Sandwich |
| 灵敏度 | 0.91ng/mL |
| 检测范围 | 2-600ng/mL |
| 保存条件 | Store at 2 to 8 degree C for 6 months. Inter-assay Precision Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10% Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8% |
【产品特点】
-
检测对象:Complement 4(Monkey)。
-
检测方法:Quantitative Sandwich。
-
灵敏度:0.91ng/mL。
-
样本类型:Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids。
-
质量体系:说明书标注产品在通过 ISO 13485/ISO 9001 认证的实验室生产。
【应用参考】
标准曲线与理论检测范围请参考随附说明书;实际样本浓度需经标准曲线插值计算。
【保存与使用】
Store at 2 to 8 degree C for 6 months. Inter-assay Precision Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10% Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%。本试剂盒仅供科研使用,不用于临床诊断或治疗。
【订购咨询】
欢迎提供货号 MBS1604166-48T 及所需采购量,咨询包装规格、报价、交期和批次资料。
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文献和实验【靶点相关参考文献】
以下文献/资料介绍 Complement 4 的生物学背景或相关研究,供研究参考;不代表这些文献使用了 MBS1604166-48T 产品。
-
Diseases associated with C4 elisa kit
-
DISEASE NAME PUBMED PUBLICATIONS
-
Skin Diseases Antibodies >1200 publications with C4 and Skin Diseases
-
Female Urogenital Diseases Antibodies >1128 publications with C4 and Female Urogenital Diseases
-
Inflammation Antibodies >1083 publications with C4 and Inflammation
-
Lung Diseases Antibodies >825 publications with C4 and Lung Diseases
-
Asthma Antibodies >654 publications with C4 and Asthma
-
Spinal Cord Injuries Antibodies >508 publications with C4 and Spinal Cord Injuries
-
Edema Antibodies >413 publications with C4 and Edema
-
Carcinoma Antibodies >410 publications with C4 and Carcinoma
-
Drug Toxicity Antibodies >360 publications with C4 and Drug Toxicity
-
Prostatic Neoplasms Antibodies >314 publications with C4 and Prostatic Neoplasms
-
Organs/Tissues associated with C4 elisa kit
-
ORGAN/TISSUE NAME PUBMED PUBLICATIONS
-
Other Antibodies >4058 publications with C4 and Other
-
Liver Antibodies >1069 publications with C4 and Liver
-
Heart Antibodies >613 publications with C4 and Heart
-
Limb Antibodies >423 publications with C4 and Limb
-
Head Antibodies >382 publications with C4 and Head
-
Spleen Antibodies >192 publications with C4 and Spleen
-
Small Intestine Antibodies >160 publications with C4 and Small Intestine
-
Testis Antibodies >141 publications with C4 and Testis
-
Embryonic Tissue Antibodies >122 publications with C4 and Embryonic Tissue
-
Pancreas Antibodies >77 publications with C4 and Pancreas
【分享】Human press METHODS IN MOLECULAR BIOLOGY
and Protocols. Edited by Pascol Bailon, 2000 148.DNA-protein interactions, Principle and protocols, 2nd, Edited by Tom Moss, 2000 149.The ELISA guide book, Edited by John Crowther, 2000 150.Complement, Methods and protocols. Edited by B. Paul Morgan. 2000
、C)广泛表达于一切有核细胞表面,II类抗原(HLA-DR、DQ、DP)主要表达在活化的巨噬细胞、B细胞、树突状细胞等抗原提呈细胞、血管内皮细胞及活化的T细胞表面。自体移植和同种同基因的移植不发生排斥。 在同种异基因移植或异种移植中,移植成功的关键取决于供、受者间组织相容性抗原是否一致或相近。根据排斥反应发生的时间、强度及病理学改变可分为超急性排斥、急性排斥和慢性排斥反应。①超急性排斥反应(hyperacute rejection)是在移植物血液循环恢复后数分钟或数小时(也可在24~48h
1、CD8+细胞、NK细胞产生IFN-γ来间接抑制NK细胞的活性。如B细胞淋巴瘤细胞可分泌IL-10,能降低DC的生长速度和活性[26]; Chen-Q等用PCR和ELISA方法对一些黑色素瘤细胞来进行检测,发现其中的66%的细胞系可检出IL-10的mRNA,其培养上清中可检出IL-10。当用抗IL-10的单抗中和其中的因子后,可以消除肿瘤培养上清对淋巴细胞产生IFN-α、IFN-γ和IL-2等细胞因子的抑制作用。此外,荷瘤宿主体内的T细胞和巨噬细胞较正常宿主的淋巴细胞对IL-10的抑制作用更为敏感
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