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文献和实验Comparative Protein Structure Modeling Using MODELLER
of a protein sequence is a common goal in biology, and is usually facilitated by having an accurate three?dimensional (3?D) structure of the studied protein. In the absence of an experimentally determined structure, comparative or homology modeling
Strategies to Optimize Protein Expression in E. coli
:2‐18. Arnold, K., Bordoli, L., Kopp, J., and Schwede, T. 2006. The SWISS‐MODEL workspace: A web‐based environment for protein structure homology modelling. Bioinformatics 22
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
) (Abcam, Cambridge, UK; Sigma, St. Louis, USA.; Roche, Mannheim, Germany). We recently generated a GFP-binding protein (GBP) based on a single domain antibody derived from Lama alpaca (Rothbauer et al., 2008). This GFP-binding protein is characterized
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