PHR1061-1G默克Merck Sigma-Aldric

Loss of Heterozygosity

Elmer)a-32P dCTP, 6000 Ci/mmol (NEN Dupont)Formamide, 99% (Fluka)Bromophenol blue-Xylene cyanole (Sigma), reconstituted as directedGel Mix-6 sequencing gel solution (Life Technologies)Ammonium persulfate (Biorad)10X TBE buffer (0.89 M Tris Base, 0.89 M

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

acetonitrile, 99.8% (CH 3 CN, Sigma‐Aldrich) Ribonucleoside or 2′‐deoxyribonucleoside 5′‐triphosphate standard (Epicentre

Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF

Bicarbonate 2.93 gSodium Carbonate 1.71 gDistilled H2O up to 1 literWASHING BUFFER10% Tween 20 ml10 mM PBS 4 litersBLOCKING BUFFER10 mM PBS 99 mlFCS 1 mlSAMPLE BUFFER0.5% BSA (Albumin Bovine) 2.5 g.0001% or 1 µg/ml Aprotinin .0005 g0.1 mM Benzethonium Chloride