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现货
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现货
- 供应商:
上海然其生物科技有限公司
- 规格:
1块/包/60包/箱
| 规格: | 1块/包 | 产品价格: | ¥67.98 |
|---|---|---|---|
| 规格: | 60包/箱 | 产品价格: | ¥4079.0 |
NUNC 278743 U96 DeepWell 96 孔 2.0mL 深孔板(已灭菌)
中文介绍
货号:278743 品牌:Thermo Scientific™ Nunc™ 名称:Nunc DeepWell™ 96 孔聚丙烯深孔板,U 型圆底,2.0mL,无盖板,γ 辐照灭菌
基础参数
- 材质:医用原生聚丙烯 PP(符合 USP <661.1>),本色半透明
- 孔型:96 孔,U 型圆底(Round U-bottom),非细胞培养表面(未 TC 处理)
- 单孔总容积:2.0 mL;推荐工作体积:50–1900 μL
- 板外尺寸:128 mm × 86 mm,ANSI/SBS 标准,适配自动化移液工作站、磁珠提取、封板设备
- 工艺:共壁 Shared-Wall 技术,板身更低,孔内径更大,混匀效果好、液体残留少
- 温度耐受:-80℃ ~ +121℃,可高压灭菌
- 灭菌:γ 辐照灭菌,SAL=10⁻⁶;无 RNase、无 DNase、无热原、无人类 DNA、PCR 抑制剂阴性
- 配套:不含盖板,可单独选配封板垫 / 封板膜 / 封板盖
- 包装规格:60 块 / 整箱
产品特点
- Shared-Wall 共壁设计,降低板体高度,增大单孔内径;U 型圆底,磁珠回收效率高、液体残留少,样本转移回收率更高
- 聚丙烯材质耐 DMSO、乙醇、异丙醇等有机溶剂,化学稳定性强,适合化合物库、有机试剂体系
- 低 DNA 吸附特性,减少核酸样本吸附损耗,适合 NGS、PCR、核酸提取、多组学样本分装储存
- 分子级洁净生产,无核酸酶、无热原,适配高通量自动化液体处理平台
- 可搭配热封膜、自粘封板膜、硅胶封板垫多种密封方案,降低样本挥发与交叉污染风险
适用场景
高通量样本收集、核酸 / 蛋白样本分装与低温储存、磁珠法核酸提取、化合物库构建与保存、高通量筛选、细菌 / 酵母培养、液相反应体系、NGS 建库样本前处理。
⚠️重要区分:无 TC 细胞培养表面,不适合贴壁细胞培养;278743 为灭菌款;同型号非灭菌货号 278752。
English Introduction
Catalog No.: 278743 Brand: Thermo Scientific™ Nunc™ Name: Nunc DeepWell™ 96-well Polypropylene DeepWell Plate, U round bottom, 2.0 mL, sterile, without lid
Specifications
- Material: Virgin medical-grade Polypropylene (PP), meets USP <661.1>, natural translucent
- Well type: 96 wells, U-shaped round bottom, non-tissue culture treated surface
- Total well volume: 2.0 mL; recommended working volume: 50–1900 μL
- Outer dimension: 128 mm × 86 mm, ANSI/SBS compliant, compatible with liquid handlers, magnetic bead extraction and plate sealers
- Technology: Shared-wall construction
- Temperature range: -80 ℃ ~ +121 ℃, autoclavable
- Sterilization: Gamma irradiation, SAL=10⁻⁶; RNase-free, DNase-free, non-pyrogenic, human DNA-free, free of PCR inhibitors
- Accessories: Supplied without lid, compatible with sealing mats, adhesive or heat sealing films
- Packaging: 60 plates per case
Key Features
- Shared-wall design reduces plate height and increases well diameter. U round bottom minimizes liquid retention and improves magnetic bead recovery.
- Polypropylene resin is chemically resistant to DMSO, ethanol, IPA and common organic solvents, suitable for compound library storage.
- Low DNA-binding property reduces nucleic acid loss, ideal for NGS, PCR, nucleic acid extraction and multi-omics sample aliquoting.
- Manufactured under molecular-grade clean conditions, nuclease-free and endotoxin-free, fully compatible with high-throughput automated liquid handling systems.
- Multiple sealing options (heat seal, adhesive film, silicone cap mat) available to reduce evaporation and cross-contamination.
Applications
High-throughput sample collection, aliquoting & cold storage of nucleic acid/protein samples, magnetic bead-based nucleic acid extraction, compound library banking, high-throughput screening, bacterial & yeast culture, liquid-phase reactions, NGS library pre-processing.
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文献和实验- Synthesis and direct assay of large macrocycle diversities by combinatorial late-stage modification at picomole scale,Nature Communications, 2022
Methods: The reaction mixtures were collected in Nunc 2.0 mL U-bottom polypropylene DeepWell 96-well plates for washing and compound library storage. 用途:大环化合物组合化学合成、化合物库分装保存,适配 DMSO 等有机试剂
- Impact of SARS-CoV-2 RBD Mutations on the Production of a Recombinant RBD Fusion Protein in Mammalian Cells,Biomolecules, 2023
Methods: Collected protein samples were transferred and stored in Nunc 2.0 mL U-bottom DeepWell polypropylene 96-well plates after chromatographic separation. 用途:重组蛋白样本收集、分装与低温暂存
- High-throughput magnetic bead-based nucleic acid extraction for multi-omics biobank samples,Clinical Chemistry and Laboratory Medicine,2024
Methods: Biosamples were lysed and processed in Nunc 2.0 mL U-bottom DeepWell 96-well plates for automated magnetic bead nucleic acid purification. 用途:生物样本库高通量磁珠法核酸提取,多组学样本前处理(与你整套肿瘤样本实验链路匹配)
时间长,可加入penicillin(50u/ml)至10x stock solution或加入thallium acetate (1:2000)至培养基中以防止其它细菌污染。 · 10x stock solution (1 liter) :称取50 g dextrose,10 g L-arginine HCl,溶于1 liter蒸馏水中。以0.22μm无菌过滤膜过滤灭菌。分装100 ml至瓶中,保存于 -70℃。 · 液体培养基 (1 liter) :称取21 g之Difco PPLO
.1.1)。 ( 9 ) PEG/NaCl ( 见 12.2.1.1)。 ( 10 ) SOC 培养基:5 g 细菌用酵母抽提物、20 g 细菌用胰蛋白胨和 0.5 g 氯化钠及 0.2 g 氯化钾;加水到大约1 L,用氢氧化钠调节 pH 到 7.0;高压灭菌;然后加入 5 ml 2.0 mol/L 高压灭菌过的氯化镁溶液及 20 ml 超滤灭菌过的 1.0 mol/L 葡萄糖溶液;并将最终体积调至 1 L。 ( 11 ) M13KO7 辅助噬菌体(见 12.2.1.1)。 2.1.4 制备电穿孔法感受
) 。 ( 3 ) 384 孔微量滴定板(Gentix,Christchurch,UK ) 。 ( 4 ) 96 孔深孔板:每孔容积 2 ml 的 96 孔微滴定板(Qiagen,Hilden,Germany) 。 ( 5 ) 96 针复制器(Nunc,Wiesbaden,Germany) 。 ( 6 ) 4 X SB 培养基:48 g/L 胰化蛋白胨,96 g/L 酵母提取物,0.8% ( V/V)甘油,120°C 高压灭菌 20 min。 ( 7 ) 20 X PP 缓冲液(磷酸钾盐缓冲
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