Anti-Glypican 3 抗体 [SP86]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

SP86

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt, ICC/IF, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/80", "FlowCyt-species-notes": "

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "

", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "

Perform heat mediated antigen retrieval (boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min).

", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "

" } } }

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

• Visit the General protocols
• Visit the Troubleshooting

产品详情

What is this antibody validated in?
Anti-Glypican 3 antibody [SP86] (ab95363) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Glypican 3?
Anti-Glypican 3 [SP86] (ab95363) specifically detects a band for Glypican 3 (UniProt: P51654) at a molecular weight of 66kDa.

Collaboration
Anti-Glypican 3 [SP86] (ab95363) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Specificity confirmed
The specificity of Anti-Glypican 3 antibody [SP86] (ab95363) has been confirmed by Western blot testing in GPC3 Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [SP86] also available for your convenience: ab95363, Carrier free - ab238804, Alexa Fluor® 647 - ab282189, PE - ab303587, Alexa Fluor® 488 - ab314880

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

• - High batch-to-batch consistency and reproducibility
• - Improved sensitivity and specificity
• - Long-term security of supply
• - Animal-free batch production

For more information, read more on recombinant antibodies.

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

运输条件

Conditional Ambient

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

靶点信息

Cell surface proteoglycan (PubMed : 14610063). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PubMed : 16227623, PubMed : 24496449). Positively regulates the non-canonical Wnt signaling pathway (By similarity). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity). Inhibits the dipeptidyl peptidase activity of DPP4 (PubMed : 17549790). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity). Required for coronary vascular development (By similarity). Plays a role in regulating cell movements during gastrulation (By similarity).

See full target information GPC3