Anti-Integrin alpha V + beta 6

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

10D5

亚型

IgG2a

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

IP, Flow Cyt, FuncS (Neut/Block)

applications

免疫原

Cell preparation containing Itgav protein. The exact immunogen used to generate this antibody is proprietary information.

P43406

特异性

ab77906 recognises alpha V beta 6 and does not recognize alpha V or other alpha V integrins.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "

because this antibody is an IgG2a, protein G or rabbit anti mouse IgG is helpful for capture.

", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "

Inhibition of alpha V beta 6 mediated cell adhesion and cell migration are achieved at a concentration of 100 μg/mL using beta 6 transfected cells tested against TGF beta LAP.

", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "

Flow cytometry should be done with buffers containing 1 mM Mg, since the epitope recognized by this antibody is divalent cation dependent. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "

", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "

" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "

because this antibody is an IgG2a, protein G or rabbit anti mouse IgG is helpful for capture.

", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "

Inhibition of alpha V beta 6 mediated cell adhesion and cell migration are achieved at a concentration of 100 μg/mL using beta 6 transfected cells tested against TGF beta LAP.

", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "

Flow cytometry should be done with buffers containing 1 mM Mg, since the epitope recognized by this antibody is divalent cation dependent. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "

", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "

" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "

because this antibody is an IgG2a, protein G or rabbit anti mouse IgG is helpful for capture.

", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "

Inhibition of alpha V beta 6 mediated cell adhesion and cell migration are achieved at a concentration of 100 μg/mL using beta 6 transfected cells tested against TGF beta LAP.

", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "

Flow cytometry should be done with buffers containing 1 mM Mg, since the epitope recognized by this antibody is divalent cation dependent. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "

", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "

" } } }

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

• Visit the General protocols
• Visit the Troubleshooting

产品详情

Product Specifications
Anti-Integrin alpha V + beta 6 antibody [10D5] - BSA and Azide free (ab77906) is a mouse monoclonal antibody provided in a carrier-free, conjugation ready format.
ab77906 is validated in Flow Cyt, IP in human, mouse, rat samples.
Carrier-free antibodies are compatible with our best-in-class range of Lightning-Link® conjugation kits.
Anti-Integrin alpha V + beta 6 antibody [10D5] - BSA and Azide free (ab77906) specifically detects Integrin alpha V + beta 6 (UniProt ID: P06756; Molecular weight: 116kDa) and is sold in 50 µg selling sizes.

Quality and Validation
Antibody clone 10D5 has been cited over 19 times in peer reviewed journals and is trusted by the scientific community.
Anti-Integrin alpha V + beta 6 antibody [10D5] - BSA and Azide free (ab77906) has 5 independent reviews from customers.

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

纯化说明

Sterile filtered

存储溶液

Constituents: PBS

运输条件

Blue Ice

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

靶点信息

The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV : ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 and ITGAV : ITGB6 act as receptors for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887, PubMed : 17158881). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV : ITGB6 or ITGAV : ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed : 15184403, PubMed : 22278742, PubMed : 28117447). ITGAV : ITGB3 acts as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428).. (Microbial infection) Integrin ITGAV : ITGB5 acts as a receptor for Adenovirus type C.. (Microbial infection) Integrin ITGAV : ITGB5 and ITGAV : ITGB3 act as receptors for Coxsackievirus A9 and B1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB6 acts as a receptor for herpes simplex 1/HHV-1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

See full target information ITGAV

其他靶点

Integrin beta-6