Anti-DYRK1A抗体

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human DYRK1A aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q13627

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "

", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "

" } } }

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

• Visit the General protocols
• Visit the Troubleshooting

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab65220 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

运输条件

Blue Ice

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

储存信息

Stable for 12 months at -20°C

靶点信息

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities (PubMed : 20981014, PubMed : 21127067, PubMed : 23665168, PubMed : 30773093, PubMed : 8769099). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3 (PubMed : 23665168). Plays an important role in double-strand breaks (DSBs) repair following DNA damage (PubMed : 31024071). Mechanistically, phosphorylates RNF169 and increases its ability to block accumulation of TP53BP1 at the DSB sites thereby promoting homologous recombination repair (HRR) (PubMed : 30773093). Also acts as a positive regulator of transcription by acting as a CTD kinase that mediates phosphorylation of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A (PubMed : 25620562, PubMed : 29849146). May play a role in a signaling pathway regulating nuclear functions of cell proliferation (PubMed : 14500717). Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Has pro-survival function and negatively regulates the apoptotic process (By similarity). Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1 (By similarity). This in turn inhibits p53/TP53 activity and apoptosis (By similarity). Phosphorylates SEPTIN4, SEPTIN5 and SF3B1 at 'Thr-434' (By similarity).

See full target information DYRK1A