- ￥10413
- Biorbyt
- orb344676
- 英国
- 2026年08月12日
- ELISA, WB
- Rabbit
- Bacteria
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- 技术资料
- 抗体名：
ospB Antibody 抗体
- 抗体英文名：
ospB Antibody
- 靶点：
ospB
- 浓度：
1.0 mg/mL
- 应用范围：
ELISA, WB
- 宿主：
Rabbit
- 适应物种：
Bacteria
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb344676
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Lyophilized
- 亚型：
IgG
- 免疫原：
详询
- 规格：
100 ug
类别: Antibodies
产品子类型: Primary Antibody
描述: OspB antibody
别名: rabbit anti-OspB Antibody, Outer surface protein B, Borrelia burgdorferi OspB, locus BB_A16
应用稀释比例: ELISA: 1:13,000, WB: 1:1,000
应用说明: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 30.3 kDa in size corresponding to Borrelia burgdorferi OspB protein by Western blotting in the appropriate cell lysate or extract.
免疫原: MBP-fusion protein corresponding to Borrelia burgdorferi OspB protein.
防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
纯度: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspB protein. A BLAST analysis was used to suggest cross-reactivity with OspB from B. burgdorferi, afzelii, spielmanii, and garinii sources based on 100% homology with the immunizing sequence, and with B. valaisiana based on 99% homology. Cross-reactivity with OspB from other sources has not been determined.
UniProt ID: P17739
NCBI 参考序列: WP_010890379.1
保存条件: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
备注: For research use only.
产品子类型: Primary Antibody
描述: OspB antibody
别名: rabbit anti-OspB Antibody, Outer surface protein B, Borrelia burgdorferi OspB, locus BB_A16
应用稀释比例: ELISA: 1:13,000, WB: 1:1,000
应用说明: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 30.3 kDa in size corresponding to Borrelia burgdorferi OspB protein by Western blotting in the appropriate cell lysate or extract.
免疫原: MBP-fusion protein corresponding to Borrelia burgdorferi OspB protein.
防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
纯度: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspB protein. A BLAST analysis was used to suggest cross-reactivity with OspB from B. burgdorferi, afzelii, spielmanii, and garinii sources based on 100% homology with the immunizing sequence, and with B. valaisiana based on 99% homology. Cross-reactivity with OspB from other sources has not been determined.
UniProt ID: P17739
NCBI 参考序列: WP_010890379.1
保存条件: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
备注: For research use only.
Western blot showing detection of 0.1 µg of recombinant OspB protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspB fusion protein (arrow; expected MW = 72.7 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n orb348540, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n orb347654) secondary antibody was used at 1:40000 in orb348637 blocking buffer.
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ospB Antibody 抗体，orb344676，Biorbyt
￥10413
