TWEAK/TNFSF12, Mouse, Recombin

TWEAK/TNFSF12, Mouse, RecombinantTWEAK/TNFSF12, Mouse, RecombinantTNFSF12;DR3LG;Apo3 Ligand;AI255180;C87282;Fn1;Fn14;HPIP;TWEAK-R;Twea;TweakR;Tnfrsf12a

TWEAK/TNFSF12, Mouse, Recombinant PCK331

背景介绍：TWEAK belongs to the TNF family of ligands, and also known as TNFRSF12A through TWEAK receptor signaling. TWEAK wildly expresses in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK has the ability to trigger activation and chemokine secretion of NF-κB, and to apply an apoptotic activity in certain cells, such as HT-29 human cultured adenocarcinoma cells feeding with IFN-γ. TWEAK also promotes proliferation and migration of endothelial cells.

产品形态	粉末
产品规格	10μg
别名	TNFSF12;DR3LG;Apo3 Ligand;AI255180;C87282;Fn1;Fn14;HPIP;TWEAK-R;Twea;TweakR;Tnfrsf12a
物种	小鼠
表达宿主	E.coli
序列信息	MSAPKGRKARPRRAIAAHYEVHPRPGQDGAQAGVDGTVSGWEETKINSSSPLRYDRQIGEFTVIRAGLYYLYCQVHFDEGKAVYLKLDLLVNGVLALRCLEEFSATAASSPGPQLRLCQVSGLLPLRPG SSLRIRTLPWAHLKAAPFLTYFGLFQVH with polyhistidine tag at the C-terminus.
检索号	Q9CR75.1
分子量	18.14 kDa
表观分子量	17-25 kDa
标签	His-tag at the C-terminus
生物活性	Measure by its ability to induce proliferation in HUVEC cells. The ED50 for this effect is < 0.2 μg/mL.
纯度	> 98% as determined by SDS-PAGE. Ni-NTA chromatography.
内毒素	< 0.1 EU/μg
储存条件	Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
运输条件	Ambient temperature or ice pack.
有效期	12个月
制剂	The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4.
复融	It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
体系描述	GMP生产和质量体系 公司符合cGMP的要求，已通过NMPA的第一类医疗器械生产备案，且通过ISO 13485和ISO 9001标准认证。公司生产的TWEAK/TNFSF12, Mouse, Recombinant符合ISO 9001的cGMP标准。

产品问答FAQs：

Q：细胞因子离心前需要放置室温吗？

A：不用提前放置室温.

Q：细胞因子加无菌水溶解后需要再次离心吗？

A：加了无菌水后，如果没有挂壁现象是不用离心的，如果您放置了一段时间，再次使用时是可以使用离心机离心的

Q：细胞因子用无菌水配成了100ug/mL母液，用于实验时，需要的终浓度为10ng/mL，可以用培养基直接稀释？还是需要通过PBS（含5%海藻糖）进一步稀释才行呢？

A：如果是直接做实验，可以直接添加培养基进行稀释配置。 如果是需要长时间保存的话，需要使用含载体蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液进一步释后分装保存于-5~-20℃或者-80℃冰箱

普诺赛®提供的细胞因子专为细胞培养和研究而设计，经过严格的质量控制以及细胞功能学的测试和验证，确保产品具有高纯度、高生物学活性和低类毒素水平，同时有人、大鼠、小鼠和猪等多个种属因子可供选择，适用于多种细胞的培养及功能研究。普诺赛®专注于细胞培养及相关领域的研究，可提供质量稳定可靠，不含动物源成分的重组细胞因子，欢迎咨询选购。

TWEAK/TNFSF12, Mouse, Recombinant Ambient temperature or ice pack. Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. 12个月