- ￥2890
- AGRISERA
- 瑞典
- AS10 1075
- 2026年08月07日
- WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- Rabbit
- Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
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- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型：
Rabbit IgG
- 形态：
Lyophilized
- 保存条件：
-20°C.
- 克隆性：
Polyclonal
- 标记物：
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种：
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期：
long term storage (1 year or more) at -20°C.
- 抗原来源：
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别：
科研
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度：
Concentration after reconstitution: 1 µg/µL
- 抗体英文名：
Rat IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse IgG
- 抗体名：
Goat anti-Rat IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse IgG
- 规格：
1 mg
Goat anti-Rat IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse IgG
AS10 1075| Clonality:Polyclonal| Host:Goat| Reactivity:Rat IgG (H&L)
Product Info
| Host: | Goat |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified IgG. |
| Format: | Liquid |
| Quantity: | 1 mg |
| Storage: | Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilution prior to use and then discard. Shelf life of this product is one year from date of receipt. |
| Recommended dilution: | 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB) |
Reactivity
Additional Information
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or to human or mouse IgG based on immunoelectrophoresis. Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Background
Goat anti-rat IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are adsorbed against human,mouse IgG and affinity purified using solid phase rat IgG.
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
blots. In direct ELISAs, this antibody shows less than 2% cross-reactivity with human IgG and rabbit IgG. Optimal dilutions should be determined by each laboratory for each application. 谢谢!!!
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