- ￥3550
- AGRISERA
- 瑞典
- AS05 066
- 2026年08月07日
- WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- Rabbit
- Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
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- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型：
Rabbit IgG
- 形态：
Lyophilized
- 保存条件：
-20°C.
- 克隆性：
Polyclonal
- 标记物：
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种：
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期：
long term storage (1 year or more) at -20°C.
- 抗原来源：
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别：
科研
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度：
Concentration after reconstitution: 1 µg/µL
- 抗体英文名：
small subunit X of PSII
- 抗体名：
Anti-PsbX
- 规格：
200 µl
Anti-PsbX | small subunit X of PSII
AS05 066| Clonality:Polyclonal| Host:Rabbit| Reactivity:Arabidopsis thaliana
Product Info
| Immunogen: | KLH-conjugated synthetic peptide dervied from Arabidopsis thaliana PsbX sequence UniProt: At2g06520 |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Serum |
| Format: | Lyophilized |
| Quantity: | 200 µl |
| Reconstitution: | For reconstitution add 200 µl of sterile water |
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | Western blot (WB) |
| Recommended dilution: | 1 : 2500, 1 µg of chlorophyll/lane (WB) |
| Expected | apparent MW: | 4 kDa |
Reactivity
| Confirmed reactivity: | Arabidopsis thaliana |
| Predicted reactivity: | Dictos, Oryza sativa, Zea mays Species of your interest not listed? Contact us |
| Not reactive in: | Cyanobacteria |
Additional Information
PsbX is a small (4 kDa) and very hydrophobic subunit of PSII, Immunoblots from SDS-gels (especially with high loading) may show higher molecular weight signals from PsbX not fully detached from other subunits of PSII, The use of 15% SDS-PAGE gels with 2-4 M urea is recommended when working with this antibody Additional information (application): For an image of antibody detection in a western blot application see Garcia-Cerdan et al. (2008).
Background
Plant PsbX is a nucelar encoded small 4 kDa protein associated with Photosystem II and found in all classes of oxygenic organisms.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
and additions: 1.To make saturated culture combine 5 ml of YPD with 25µl of 0.4% adenine and 1 blob of yeast.Incubate at 30℃ for 18 to 24 hr until yeast is at a density of 2.0 x108 cells/ml. 2.Grow cells to an OD600 of ~1.0 after inoculating 1 liter of YPD
Purification of TFIIIC from yeast
and additions: 1.To make saturated culture combine 5 ml of YPD with 25µl of 0.4% adenine and 1 blob of yeast.Incubate at 30℃ for 18 to 24 hr until yeast is at a density of 2.0 x108 cells/ml. 2.Grow cells to an OD600 of ~1.0 after inoculating 1 liter of YPD
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