
Plant Phosphoenolpyruvate carb
oxylase Antibody 植物抗体(Anti-PEPC)相关产品推荐更多 >
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- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型:
Rabbit IgG
- 形态:
Lyophilized
- 保存条件:
-20°C.
- 克隆性:
Polyclonal
- 标记物:
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期:
long term storage (1 year or more) at -20°C.
- 抗原来源:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别:
科研
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度:
Concentration after reconstitution: 1 µg/µL
- 抗体英文名:
Phosphoenolpyruvate carboxylase
- 抗体名:
Anti-PEPC
- 规格:
50 µg
Anti-PEPC | Phosphoenolpyruvate carboxylase
AS09 458| Clonality:Polyclonal| Host:Rabbit| Reactivity:A. comosus, A. thaliana,C. ciliaris, C. gayana, C. velia, Ch. quinoa, H. vulgare, J. curcas, K. prostrata, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharumspp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi,Z. mays, Z. muelleriBenefits of using this antibody
Product Info
| Immunogen: | KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4). For Zea mays, the peptide is converved in PEP1 and PEP4 isoforms. |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified serum in PBS pH 7.4. |
| Format: | Lyophilized |
| Quantity: | 50 µg |
| Reconstitution: | For reconstitution add 50 µl of sterile water |
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Please do not re-use this primary antibody solution. In case of cyanobacterial samples there will be no signal in your second incubation. |
| Tested applications: | Immunolocalization (IL), Western blot (WB) |
| Recommended dilution: | 1 : 500 (IL), 1: 1000 - : 10 000 (WB) |
| Expected | apparent MW: | 110 | 105 kDa |
Reactivity
| Confirmed reactivity: | Ananas comosus, Arabidopsis thaliana, Cenchrus ciliaris, Chenopodium quinoa, Chloris gayana, Chromera velia, Cyanthobasis fruticulosa, Glycine max, Hordeum vulgare, Jatropha curcas, Kochia prostrata, Leptochloa fusca, Lupinus sp. , Megathyrsus maximus, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Panicum antidotale, Panicum coloratum, Petrosimonia nigdeensis, Pinus strobus, Saccharum spp. hybrid clone C91-301, Salsola lanata, Salsola laricifolia,Salsola grandis, Salsola tragus Sorghum bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), Pinus strobus, Thalassiosira weissfloggi, Zea mays, Zostera muelleri |
| Predicted reactivity: | Amaranthus sp., Brassica napus, Chlorella ohadi, Cucumis sativus (PEPC1, PEPC2, PEPC3), Flaveria bidentis, Flaveria trinervia, Lupinus albus, Mammillaria thornberi, Manihot esculenta, Manihot obovata, Medicago sativa, Morinda citrifolia, Nannochloropsis gaditana CCMP526, Nopalea gaumeri, Opuntia macbridei, Pachycereus pringlei, Pachycereus hollianus, Pisum sativa, Phaseolus vulgaris, Phragmites australis, Populus sp.,Saccharum spp, Solanum tuberosum, Spinacia oleracea, Streptanthus tortuosus,Triticum aestivum, algae, diatoms: Thalassiosira pseudonana, other species: Salmonella sp., Schiedea hookeri, Shigella sp. Schiedea sarmentosa, Solanum lycopersicum, Streptanthus farnsworthianus, Tacinga saxatilis,Yersinia sp. Vibrio sp., Quercus sp. Species of your interest not listed? Contact us |
| Not reactive in: | Methanothermobacter thermautotrophicus |
Additional Information
PEPC is especially prone to proteolysis. To avoid artifacts, we use chymotrypsin, as recommended by Plaxton (2019) , in addition to the Roche protease inhibitor cocktail. Additional information (application): Antibody can be also used following 2D gel electrophoresis
Background
PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
)。C4植物的BSC中含有大而多的叶绿体,线粒体和其它细胞器也较丰富。BSC与相邻叶肉细胞间的壁较厚,壁中纹孔多,胞间连丝丰富。这些结构特点有利于MC与BSC间的物质交换,以及光合产物向维管束的就近转运。 此外,C4植物的两类光合细胞中含有不同的酶类,叶肉细胞中含有磷酸烯醇式丙酮酸羧化酶(phosphoenolpyruvate carboxylase,PEPC)以及与C4二羧酸生成有关的酶;而BSC中含有Rubisco等参与C3途径的酶、乙醇酸氧化酶以及脱羧酶。在这两类细胞中进行不同的生化
线粒体荧光探针大全:TMRM,Mitotracker,JC-1
I, II and III) and the nucleus (all others), with a total of 13 subunits for mammalian COX and 11 subunits for yeast COX. The binding specificity exhibited by our anti�OxPhos Complex IV monoclonal antibody preparations allows researchers to investigate
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