- ￥4340
- AGRISERA
- 瑞典
- AS01 008
- 2026年08月07日
- WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- Rabbit
- Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
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- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型：
Rabbit IgG
- 形态：
Lyophilized
- 保存条件：
-20°C.
- 克隆性：
Polyclonal
- 标记物：
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种：
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期：
long term storage (1 year or more) at -20°C.
- 抗原来源：
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别：
科研
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度：
Concentration after reconstitution: 1 µg/µL
- 抗体英文名：
PSI type IV chlorophyll a/b-binding protein
- 抗体名：
Anti-Lhca4
- 规格：
0.5 mg
Anti-Lhca4 | PSI type IV chlorophyll a/b-binding protein
AS01 008| Clonality:Polyclonal| Host:Rabbit| Reactivity:MonocotsanddicotsincludingA.thaliana,C. reticulata,F. margaritaSwingle,H.vulgare,Nicotiana tabacum, O. sativa, P. patens, S. lycopersicum, S.oleracea,T. aestivum, Triticale,Z.mays
Product Info
| Immunogen: | BSA-conjugated synthetic peptide derived from the Lhca4 protein ofArabidopsis thaliana UniProt: P27521, TAIR: At3g47470. This sequence is highly conserved in Lhca4 proteins of angiosperms (monocots and dicots) and gymnosperms. |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Total IgG. Protein G purified in PBS pH 7.4. |
| Format: | Lyophilized |
| Quantity: | 0.5 mg |
| Reconstitution: | For reconstitution add 100 µl of sterile water |
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | Western blot (WB) |
| Recommended dilution: | 1 : 2000-1 : 5000 (WB) |
| Expected | apparent MW: | 27.7 | 21 kDa for Arabidopsis thaliana |
Reactivity
| Confirmed reactivity: | Arabidopsis thaliana, Bryopsis corticulans, Citrus reticulata, Echinochloa crus-galli, Fortunella margarita Swingle, Hordeum vulgare, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Pisum sativum, Posidonia oceanica, Spinacia oleracea, Triticum aestivum, Triticale, Zea mays |
| Predicted reactivity: | Dicots, Gymnosperms |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
Additional Information
Additional information (application): Protein is processed into mature form ( Jansson 1999).
Background
The light-harvesting protein Lhca4 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca4 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca4 forms a heterodimer (LHCI-730) with Lhca1 that associates with the PSI core close to PsaG and PsaF. A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073.
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
) is 1mg/ml in150mM NaCl, 50% glycerol, store at ©20C. Try 3 different [s] ofDNAse mix (A,B,C) 1,2 & 3ul stock DNAse1 2 ul 1M MgCl2 ©> 100ul H2O 4)binding rxn: components titrated & optimized by gel shiftassays 2ul probe mix Xul
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